MetaGenomics SOP

1. Sample preparation-Sequencing
2. Analysis
   • Installation
   • Preprocessing of the sequencing reads
   • Read-based profiling
   • De novo assembly
   • Taxonomic annotation of contigs
   • Functional annotation of contigs
   • Binning
   • Phylogenomic tree construction of the MAG

MetaTrasncriptomics SOP

1. Sample preparation-Sequencing
2. Analysis
   • Installation
   • Preprocessing of the sequencing reads
   • =Reference-guided analysis=
     • Read alignment
     • Transcriptional expression analysis
     • Differential expression analysis
   • =Reference-independent analysis=
     • Transcript de novo assembly
     • Transcript taxonomy classification
     • Read-based profiling

MetaProteomics SOP

1. Sample preparation-Sequencing
2. Analysis
   • Installation
   • Preprocessing of the sequencing reads
   • Reads alignment
   • Ribosome profiling
   • Small protein prediction
   • Translational quantification
   • Differential expression analysis
   • Read-based profiling

PROTOCOLS Page

MetaGenomics SOP

1. Sample preparation-Sequencing
2. Analysis
   • Installation
   • Preprocessing of the sequencing reads
   • Read-based profiling
   • De novo assembly
   • Taxonomic annotation of contigs
   • Functional annotation of contigs
   • Binning
   • Phylogenomic tree construction of the MAG

MetaTrasncriptomics SOP

1. Sample preparation-Sequencing
2. Analysis
   • Installation
   • Preprocessing of the sequencing reads
   • =Reference-guided analysis=
     • Read alignment
     • Transcriptional expression analysis
     • Differential expression analysis
   • =Reference-independent analysis=
     • Transcript de novo assembly
     • Transcript taxonomy classification
     • Read-based profiling

MetaProteomics SOP

1. Sample preparation-Sequencing
2. Analysis
   • Installation
   • Preprocessing of the sequencing reads
   • Reads alignment
   • Ribosome profiling
   • Small protein prediction
   • Translational quantification
   • Differential expression analysis
   • Read-based profiling